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DNA Sequencing Protocols by Colin A. Graham,

DNA Sequencing Protocols by Colin A. Graham,
DNA Sequencing Protocols, Second Edition, empowers researchers with the tools and knowledge required to become a sequencing expert. It covers a wide range of DNA sequencing technologies in common usage in molecular biology laboratories. It acts as a laboratory guide to DNA sequencing and it helps students and researchers to understand the instrumentation (ABI, ALF & LiCor) and chemistries involved. The reader is also given internet addresses for Genome sequencing sites and step by step guides to the use of sequence analysis software and databases i.e. for BLAST and NIX searches. The book also reviews some of the instrumentation available for automation of DNA sequencing processes which is in more widespread use in many research groups. Some of the protocols covered in detail include, M13 cloning and sequencing; Primer design; PCR product sequencing; Cycle sequencing; Solid phase sequencing; Shotgun sequencing; Fluorescent sequencing for 310, 373, 377, ALF, LiCor; Heterozygote mutation detection; Automation, and; Sequence databases and the internet. The book will also be useful to clinicians with research interests to update them on current DNA sequencing methods, applications and future prospects. DNA sequencing has become an essential tool for molecular diagnostics with the completion of many genome project.



Analytical Tools for DNA, Genes and Genomes
Analytical Tools for DNA, Genes and Genomes
An exhaustive guide to the handling, interpretation, and application of information contained in the sequence of DNA, this resource is an essential tool for academic, industrial, and private researchers in the field of life sciences. Information on applying DNA sequence information in genetics, using DNA sequence analysis and recognition software for DNA structural elements, and predicting and modeling higher order DNA structures is provided along with links to relevant web sites and an explanation of their corresponding interfaces. Chapters are formatted as frequently asked questions regarding structure, function, regulation, and the evolution of coding and non-coding DNA sequences, and reflects previously probed and published strategies for data mining and interpretation.



DNA-DNA hybridisation - DNA-DNA hybridization is a method in genetics to measure the degree of genetic similarity between DNA sequences. The technique is usually used to determine the genetic "distance" between two species.

DNA machine - The idea of using DNA as a material for molecular-scale construction of objects and devices was pioneered in the late 1980s by Nadrian Seeman and co-workers from New York University. DNA is used because of the numerous biological tools already found in nature that can affect DNA, and the immense knowledge of how DNA works previously researched by biochemists.

Ancient DNA - Ancient DNA can be loosely described as any DNA recovered from biological samples that have not been preserved specifically for later DNA analyses. Examples include the analysis of DNA recovered from archaeological and historical skeletal material, mummified tissues, archival collections of non-frozen medical specimens, preserved plant remains, ice and permafrost cores, and so on.

DNA bank - A DNA bank is a repository of DNA, usually used for research or criminal investigation. The NIAS DNA Bank, for example, collects the DNA of agrucultural organisms, such as rice and fish, for scientific research.



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